CD357 (AITR/GITR) Monoclonal Antibody (eBioAITR), eBioscience/CD357（AITR / GITR）单克隆抗体（eBioAITR）

货号：14-5875-80

规格：25 µg

价格：1399

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：eBioAITR

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.125 µg/test（Flow）；Assay-Dependent（ELISA)；Assay-Dependent(WB)；(IHC (P))

产品详细信息Description: The monoclonal antibody reacts with human AITR, Activation Inducible TNFR family member, an approximately 25 kDa member of the TNFR superfamily. AITR mRNA is detected in lymph node, peripheral blood leukocytes and weakly in spleen. AITR mRNA expression was upregulated within 24 hour on PMA/ionomycin or PHA stimulated PBMC. At the protein level, AITR is expressed by a small population of activated PBMC. AITR associates with TRAF1, TRAF2 and TRAF3 and induces nuclear factor NF-kappaBactivation via TRAF2. Recently TL6 (AITRL) has been reported as the ligand for AITR. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.Applications Reported: This eBioAITR antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and ELISA.Applications Tested: This eBioAITR antibody has been tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. It is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25 (+)CD4 (+) regulatory T cells. Knockout studies in mice also suggest the roleof this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

数据

CD357 (AITR/GITR) Antibody (14-5875-80) in FlowNormal human peripheral blood cells were cultured for 3 days with PHA and stained with Mouse IgG1 k Isotype Control Purified (Product # 14-4714-82) (open histogram) or Anti-Human CD357 (AITR) Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012).Total viable cells were used for analysis. CD357 (AITR/GITR) Antibody (14-5875-80)PLoS pathogens 2016 -Fig 6 CD8 T FR are higher in SIV-infected than uninfected rhesus macaques. Disaggregated cells from lymph nodes and spleen of SIV-uninfected (n = 6) and SIV-infected (n = 6) rhesus macaques were analyzed for CD8 T FR by flow cytometry. (A) Flow gating strategy to determine viable CD8 T FR (CD3+CD8+CXCR5 hi CD44 hi ) and CD8 conv. (B) Percent CD8 T FR in SIV-uninfected compared to SIV-infected rhesus macaques. (C) Percent of SIV-Gag tetramer+ CD8 T FR compared to CD8 conv. CD8 T FR and CD8 conv from SIV-uninfected and SIV-infected rhesus macaques were analyzed for percent or MFI expression of (D) Tim-3, (E) perforin, (F) IL-10, and (G) GITR. Graphs depict median and range. Statistical significance was determined by non-parametric one-way ANOVA (Friedman test) and is displayed as * = p<0.05 and *** = p<0.001. CD357 (AITR/GITR) Antibody (14-5875-80)PLoS pathogens 2016 -Fig 1 Human tonsil CD8 T FR are distinct from conventional CD8 T cells. Disaggregated tonsil cell cultures were mock-spinoculated or spinoculated with X4- or R5-tropic HIV and cultured for 2 days (n = 8). (A) Flow gating strategy to determine viable, CD8 T FR (CD3+CD8+CXCR5 hi CD44 hi ) and all other CD3+CD8+ cells (CD8 conv). (B) The percentage of CD8 T FR in mock- or HIV-spinoculated samples. The percent or MFI of CD8 T FR and CD8 conv expressing (C) Tim-3, (D) perforin, (E) IL-10, (F) IL-15 receptor, (G) GITR, and (H) CD122 (IL-2Rbeta). Graphs depict median and range. Statistical significance was determined by non-parametric one-way ANOVA (Friedman test) and is displayed as * = p<0.05 and ** = p<0.01.

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流式抗体

参考文献：

1.OncotargetCD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.2. PLoS pathogensFollicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.3.RetrovirologyHTLV-1 modulates the frequency and phenotype of FoxP3+CD4+ T cells in virus-infected individuals.4.The Journal of infectious diseasesCD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

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