Bio-Rad MCA1850 CD99抗体,mouse anti human CD99-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Bio-Rad MCA1850 CD99抗体,mouse anti human CD99

2024-10-22

mouse anti human CD99

货号：MCA1850

规格：0.2 mg

价格：3868

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD99

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：DN16

荧光染料：Purified

产品介绍
Mouse anti human CD99 antibody, clone DN16recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32 kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence "LPDNENKK" located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gilet al.2002).CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells.Functionally CD99 has been found to be involved in cellular adhesion/aggregation (Krisanaprakornkitet al.2013) and apoptosis (Sciandraet al.2014).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
Western Blotting	√		1/1000
Immunohistology - Frozen	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests
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数据
Published customer image:FITC conjugated Mouse anti human CD99 antibody, clone DN16 (MCA1850) used to evaluate CD99 expression levels on human neutrophils and monocytes by flow cytometry.Image caption:Effect of flexible ureterenoscopy on CD99 cell surface expression of neutrophils and monocytes. The points represent median ± Iqr.From: Hughes SF, Moyes AJ, Lamb RM, Ella-Tongwiis P, Snyper NYF, Shergill I.The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.Eur J Med Res. 2020 Dec 11;25(1):68.doi: 10.1186/s40001-020-00466-7.This image is from an open access article distributed under terms of aCreative Commons Attribution License.
文献
1. Choi, E.Y.et al.(1998) Engagement of CD99 induces up-regulation of TCR and MHC class I and II molecules on the surface of human thymocytes.J Immunol. 161 (2): 749-54.2. Hahn, J.H.et al.(1997) CD99 (MIC2) regulates the LFA-1/ICAM-1-mediated adhesion of lymphocytes, and its gene encodes both positive and negative regulators of cellular adhesion.J Immunol. 159 (5): 2250-8.3. Kim, S.H.et al.(1998) Generation of cells with Hodgkin's and Reed-Sternberg phenotype through downregulation of CD99 (Mic2).Blood. 92 (11): 4287-95.4. Kim, S.H.et al.(2008) Viral latent membrane protein 1 (LMP-1)-induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype.Blood. 95: 294-300.5. Husak, Z.et al.(2010) Death induction by CD99 ligation in TEL/AML1-positive acute lymphoblastic leukemia and normal B cell precursors.J Leukoc Biol. 88: 405-12.6. Husak, Z. and Dworzak, M.N. (2012) CD99 ligation upregulates HSP70 on acute lymphoblastic leukemia cells and concomitantly increases NK cytotoxicity.Cell Death Dis. 3: e425.7. Hughes, S.F.et al.(2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.Eur J Med Res. 25 (1): 68.

技术参数

产品应用 IHC(F);Flow;WB

产品类型 Monoclonal Antibody

推荐用于 IHC(F);Flow;WB

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Bio-Rad MCA1850 CD99抗体,mouse anti human CD99

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