Phospho-SLP-76 (Tyr128) Monoclonal Antibody (HNDZ55), APC

货号：17-9037-42

规格：100 Tests

价格：3202

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HNDZ55

荧光染料：APC

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), APC
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

产品详细信息Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: APC Mouse IgG2b Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: APC Mouse IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. This should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Phospho-SLP-76 (Tyr128) Antibody (17-9037-42) in FlowIntracellular staining of normal human peripheral blood cells that were unstimulated (orange histogram), stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0037-81) and goat anti-mouse IgG for 5 minutes (purple histogram), or treated with hydrogen peroxide-activated sodium pervanadate (green histogram) with Anti-Human phospho-SLP-76 (Y128) APC. The Intracellular Fixation and Permeabilization Buffer Set (88-8824) and protocol were used for staining. Lymphocytes in the CD3+ gate were used for analysis.

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