CD30 Human ELISA Kit ELISA试剂盒,CD30 Human ELISA Kit/人CD30 ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CD30 Human ELISA Kit ELISA试剂盒,CD30 Human ELISA Kit/人CD30 ELISA试剂盒

2024-10-23

CD30 Human ELISA Kit/人CD30 ELISA试剂盒

货号：BMS240,BMS240TEN

规格：96 Tests,10X96 Tests

价格：1,35475

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	0.3 ng/mL
检测范围	1.6-100 ng/mL
样本类型/体积	血清 25 μL 血浆 25 μL 上清液 25 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	5.6%
批内变异系数（CV）	4.1%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - HRP-共轭检测抗体 - SAV-HRP - 洗涤液 - 对照 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	CD30
Protein aliases	CD30, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, cytokine receptor CD30, Tumor necrosis factor receptor superfamily member 8, tumor necrosis factor receptor superfamily, member 8
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	HRP
Gene aliases	CD30, D1S166E, Ki-1, TNFRSF8
Gene ID	(Human) 943
Gene symbol	IL12A, IL12B
UniProt ID	(Human) P28908
Gene symbol	TNFRSF8

The Human CD30 ELISA quantitates Hu CD30 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD30.人CD30 ELISA定量检测人血清，血浆，缓冲液或细胞培养基中的人CD30。该检测试剂盒将专门识别天然和重组人CD30。Principle of the method 方法原理The Human CD30 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人CD30固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册Target information 靶标信息CD30 (Ki-1, TNF Receptor Superfamily Member 8) is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. CD30 is a positive regulator of apoptosis, and has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. CD30 is expressed by mononuclear cells in Hodgkin's lymphoma, Reed Sternberg cells and most Anaplastic Large Cell Lymphomas (ALCL). CD30 is also expressed by embryonal carcinomas.CD30（Ki-1，TNF受体超家族成员8）是TNF受体超家族的I型跨膜糖蛋白。使用单克隆抗体Ki-1，最初将CD30鉴定为霍奇金斯和Reed-Sternberg细胞的细胞表面抗原。 CD30的配体是CD30L（CD153）。 CD30与CD30L的结合介导多效性作用，包括细胞增殖，活化，分化和凋亡性细胞死亡。 CD30在霍奇金病和其他CD30 +淋巴瘤的病理生理中起关键作用。 CD30在胸腺阴性选择中充当共刺激分子。 CD30除了在霍奇金氏和Reed-Sternberg细胞上表达外，还在一些非霍奇金氏淋巴瘤（包括伯基特氏淋巴瘤），病毒感染的T细胞和B细胞以及活化后的正常T细胞和B细胞中发现。在T细胞中，CD30表达存在于产生Th2型细胞因子的T细胞子集和共表达CD45RO和IL4受体的CD4 + / CD8 +胸腺细胞中。 CD30（sCD30）的可溶形式用作反映Th2免疫反应的标志物。 TRAF2和TRAF5可以与该受体相互作用，并介导导致NF-κB活化的信号转导。 CD30是细胞凋亡的积极调节剂，已被证明可以限制自身反应性CD8效应T细胞的增殖潜能并保护人体免受自身免疫。已经报道了该基因的两个选择性剪接的转录变体，它们编码不同的同工型。 CD30在霍奇金淋巴瘤，里德·斯特恩伯格细胞和大多数间变性大细胞淋巴瘤（ALCL）中由单核细胞表达。 CD30也由胚胎癌表达。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

CD30 Human ELISA KitRepresentative standard curve for Human soluble CD30 ELISA

技术参数

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CD30 Human ELISA Kit ELISA试剂盒,CD30 Human ELISA Kit/人CD30 ELISA试剂盒

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