POROS™ XQ Strong Anion Exchange Resin,POROS™ XQ Strong Anion Exchange Resin/POROS™XQ强阴离子交换树脂-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

POROS™ XQ Strong Anion Exchange Resin,POROS™ XQ Strong Anion Exchange Resin/POROS™XQ强阴离子交换树脂

2024-10-23

POROS™ XQ Strong Anion Exchange Resin/POROS™XQ强阴离子交换树脂

货号：82073,82074

规格：10 mL,50 mL

价格：2589,9066

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

POROS™ XQ Strong Anion Exchange Resin is a next-generation, high-capacity, high-resolution, salt-tolerant resin that that enables >140 mg/mL dynamic binding capacity in the presence of up to 6.6 mS/cm NaCl, while delivering exceptional separation performance. A major improvement to the performance of traditional anion exchangers, POROS XQ resin sets the standard for strong anion exchange chromatography in both bind & elute or flow-through modes of operation.Features of POROS XQ resin include:• High dynamic binding capacity leading to reduced column size, smaller footprint, decreased water and buffer usage, and reduced cycling• High resolution supporting improved product yield and purity, ultimately improving the cost of goods for the molecule being produced• Robust salt tolerance allowing enhanced process design flexibility and streamlining of process flow• Low operating back pressure and linear pressure versus flow responses driving flexible scalability• Rigid polymeric bead with covalent surface chemistry for easier handling and packing, and superior physical and chemical stability enabling a more robust downstream purification processDynamic binding capacityPOROS XQ resin has a high binding capacity of more than 140 mg/mL at 5% breakthrough, over a broad range of process conditions, with superior binding capacity across a broad range of pH and salt conditions. Higher dynamic binding capacity was achieved by optimizing the base bead pore structure and surface chemistry.High resolutionUsing an optimized 50 µm particle size, POROS XQ resin maintains the superior resolving capacity of the POROS resins, overcoming the challenging impurity levels associated with high titer processes. Resolution remains consistent across a wide range of operating linear flow rates.Robust salt tolerancePOROS XQ resin has high and consistent protein capacity across a broad range of salt concentrations up to 140 mg/mL at 50 mM NaCl (6.6 mS/cm conductivity). The ability to load columns under higher conductivity reduces or helps eliminate the dilution of column feed streams or the removal of unit operations, such as tangential flow filtration.

数据：

Breakthrough BSA binding capacity vs. salt concentration and pHPoros XQ is able to reach high binding capacity under a wide range of process conditions. Dynamic binding capacity was determined by loading a 10 mg/mL BSA solution onto a 3.3 mL column until a 5% breakthrough was reached. The experiment was then repeated using different BSA solutions that varied in NaCl concentration and pH. Experimental conditions: Column: 0.46 cmD x 20 cmL; Base buffer: 20 mM Bis-Tris Propane; Flow rate: 300 cm/hr

BSA binding capacity vs. salt concentrationPOROS XQ resin delivers high and consistent protein capacity across a broad range of salt concentrations up to 150 mM NaCl. Dynamic binding capacity was determined by loading a 10 mg/mL BSA solution onto a 3.3 mL column until a 5% breakthrough was reached. The experiment was then repeated using different BSA solutions that varied in NaCl concentration and pH. Column: 0.46 cmDx 20 cmL, Buffer: 20 mM Bis-Tris Propane or Tris, Flow Rate: 300 cm/hr.

Resolution over a range of operating flow ratesPOROS XQ is capable of achieving excellent seperation using a very steep gradient and maintaining resolution at multiple flow rates. A protein mixure of holo-transferrin, ovalbumin, and soybean trypsin inhibitor was loaded onto a 3.3 mL column and then eluted using a 0-50% B gradient over 10 column volumns. The experiment was then repeated using different flow rates. Experimental conditions: Column: 0.46 cmDx 20 cmL, Buffer A: 20 mM Tris, pH 8.0, Buffer B: 20 mM Tris, 1.0 M NaCl, pH 8.0.

技术参数

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POROS™ XQ Strong Anion Exchange Resin,POROS™ XQ Strong Anion Exchange Resin/POROS™XQ强阴离子交换树脂

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