CarboxyLink™ Coupling Resin,CarboxyLink™ Coupling Resin/CarboxyLink™偶联树脂-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CarboxyLink™ Coupling Resin,CarboxyLink™ Coupling Resin/CarboxyLink™偶联树脂

2024-10-23

CarboxyLink™ Coupling Resin/CarboxyLink™偶联树脂

货号：20266

规格：25 mL

价格：3121

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific CarboxyLink Coupling Resin is for covalent immobilization of peptides or other carboxyl-containing (—COOH) molecules to a porous, beaded resin for use in affinity purification procedures.CarboxyLink Resin is crosslinked beaded agarose that has been activated with diamino-dipropylamine (DADPA) to contain long spacer arms, each with a primary amine at the end. When incubated with the resin and the carbodiimide crosslinker EDC (included in the CarboxyLink Immobilization Kit), carboxyl-containing molecules become permanently attached to the support by stable amide bonds. Once a molecule is coupled, the prepared affinity column can be used multiple times in typical protein affinity purification procedures. CarboxyLink Coupling Resins can also be used to immobilize other kinds of molecules using alternative amine-reactive crosslinking chemistries.Features of the CarboxyLink Coupling Resin:•CarboxyLink Coupling Resin—DADPA-activated crosslinked 4% beaded agarose•Efficient immobilization—Couple 1 to 2 mg of peptide per milliliter of resin (CarboxyLink Agarose Resin activated with greater than 16 µMol amine milliliter of resin; DADPA on UltraLink Support activated with greater than 40 µMol amine milliliter of resin)•Stable linkage—immoblization results in covalent attachment of carboxyl groups by amide bonds, allowing for multiple rounds of affinity purification with one batch of prepared resin•Flexible and gentle coupling conditions—immobilization reaction completed in simple MES or other non-amine and non-carboxyl, near-neutral buffer, with or without organic solvent.•Ideal for unmodified peptides—immobilizes peptides with high capacity and various orientations without steric hindrance, allowing for effective use in affinity purification of specific antibodiesBecause EDC crosslinks carboxylates to primary amines, peptides and other molecules that contain both of these functional groups will be variously polymerized as well as immobilized to the CarboxyLink Resin. For peptides whose only carboxyl and amino groups are at the termini, coupling with the CarboxyLink Kit will result in end-to-end chains of peptide that are linked by their C-terminal ends to the support. Generally, this structure provides for high capacity and low steric hindrance for use in affinity purification. However, in the case of peptides containing multiple primary amines (lysine) or carboxylates (glutamic and aspartic acid) in the middle of their sequence, the CarboxyLink Coupling Method may cause key binding sites to be blocked, resulting in high coupling capacity but poor binding capacity for affinity purification. Alternative methods for immobilization includeAminoLink Plus Coupling Resin,UltraLink BiosupportandSulfoLink Coupling Resin.

数据：

Immobilization chemistry for carboxyl-containing moleculesEDC crosslinker reacts with carboxylates to create an amine-reactive intermediate, resulting in covalent attachment to the DADPA-activated Resin (Thermo Scientific CarboxyLink Coupling Resin).

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45578.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

CarboxyLink™ Coupling Resin,CarboxyLink™ Coupling Resin/CarboxyLink™偶联树脂

相关推荐