Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), eFluor 660

货号：50-9718-41,50-9718-42

规格：25 Tests ,100 Tests

价格：1363,3513

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：MRRBY

荧光染料：eFluor 660

类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), eFluor 660
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test(Flow)

产品详细信息Description: This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (also known as mTOR, FRAP, RAFT) when phosphorylated on S2448. mTOR is a serine/threonine protein kinase that functions as an ATP and amino acid sensor as well as to balance nutrient availability with cell growth, proliferation, motility, survival, protein synthesis, and transcription. Activated mTOR increases production of enzymes necessary for glycolysis and controls the uptake of glucose and other nutrients. Increased glucose uptake and metabolism helps fulfill the energy needs for mTOR-driven cell growth and proliferation. When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of the eIF4E inhibitor, 4E-BP1. mTOR is phosphorylated at S2448 via the PI3 kinase/Akt signaling pathway and is autophosphorylated at Ser2481. Due to its critical role in regulation of cell growth, survival, and metabolism, and because it is often abnormally regulated in tumors, mTOR is under investigation as a potential target for anti-cancer therapy.Applications Reported: This MRRBY antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This MRRBY antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining Protocol: All protocols work well for this monoclonal antibody. Use of Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins allows for the greatest flexibility for detection of surface and intracellular (cytoplasmic) proteins. Use of Protocol B: One-step protocol: intracellular (nuclear) proteins is recommended for staining of transcription factors in conjunction with surface and phosphorylated intracellular (cytoplasmic) proteins. Protocol C: Two-step protocol: Fixation/Methanol allows for the greatest discrimination of phospho-specific signaling between unstimulated and stimulated samples, but with limitations on the ability to stain specific surface proteins (refer to "Clone Performance Following Fixation/Permeabilization" located in the Best Protocols Section under the Resources tab online). All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translation. A second pathway involves regulation of ribosomal S6 kinase, which affects ribosome biogenesis and translation elongation. Mutations affecting the gene results in Smith-Kingsmore syndrome.

数据

Phospho-mTOR (Ser2448) Antibody (50-9718-42) in FlowTOP: Mouse splenocytes were unstimulated (left) or stimulated with Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) plus Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) (right) for 48 hours. The cells were then intracellularly stained with Anti-Mouse CD3e PE (Product # 12-0031-82) and Anti-Human/Mouse phospho-mTOR (S2448) eFluor 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis. BOTTOM: Mouse splenocytes were unstimulated (orange histogram) or were stimulated with Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) plus Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence (green histogram) or absence (purple histogram) of the PI3 kinase inhibitor, LY294002, for 48 hours. The cells were then intracellularly stained with Anti-Mouse CD3e PE (Product # 12-0031-82) and Anti-Human/Mouse phospho-mTOR (S2448) eFluor 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. CD3e+ cells in the lymphocyte gate were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1.eLifeHighly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes."50-9718 was used in Immunohistochemistry to develop a tissue-based cyclic immunofluorescence method for highly multiplexed immuno-fluorescence imaging of formalin-fixed, paraffin-embedded specimens mounted on glass slides."AuthorsLin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK2.eLifeHighly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes."50-9718 was used in Immunohistochemistry to develop a tissue-based cyclic immunofluorescence method for highly multiplexed immuno-fluorescence imaging of formalin-fixed, paraffin-embedded specimens mounted on glass slides."AuthorsLin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45617.htm