HiPPR™ Detergent Removal Spin Columns, 0.1 mL,HiPPR™ Detergent Removal Spin Columns, 0.1 mL/HiPPR™洗涤剂去除离心柱-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

HiPPR™ Detergent Removal Spin Columns, 0.1 mL,HiPPR™ Detergent Removal Spin Columns, 0.1 mL/HiPPR™洗涤剂去除离心柱

2024-10-23

HiPPR™ Detergent Removal Spin Columns, 0.1 mL/HiPPR™洗涤剂去除离心柱

货号：88306

规格：24 columns

价格：2852

产品类型：蛋白提取/纯化

品牌：Thermo Fisher

Thermo Scientific HiPPR Detergent Removal Spin Column Kit is used to improve mass spectrometry results by efficiently removing detergents from 25 to 200 µL samples with low protein or peptide concentrations.Features of HiPPR Detergent Removal Spin Column Kit:•Optimized—removes >95% of detergent from samples with low-concentrations (1 to 100 µg/mL) of proteins or peptides•Fast—sample processing takes less than 15 minutes•Effective—eliminates detergent-interference in downstream applications like ELISA, isoelectric focusing and mass spectrometryAvailable Formats:•Resin Slurries—bulk resin supplied with empty 0.8 mL spin columns•Spin Columns—0.1 mL micro-centrifuge columns•96-well Spin Plates—pre-dispensed 96-well filter plates, compatible with centrifugation and vacuum manifold systems for manual or automated purification. The plates enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput separations.Optimized for sample concentrations of 1 to 100 µg/mL,the HiPPR (High Protein and Peptide Recovery) Detergent Removal Resin removes >95% of detergents used in protein extraction and biological sample preparation with minimal loss of protein and peptide. The Detergent Removal Resin is ideal for removing commonly used detergents, including SDS, Triton™ X-100, NP-40 and CHAPS, at concentrations of 0.5-1% and is available in pre-filled spin columns and 96-well filter spin plates for sample volumes up to 100 µL. For other sample sizes, the detergent removal resin slurry is available with empty spin columns that can be used to make custom spin columns for processing sample volumes of 25 to 200 µL.The detergents and surfactants used to prepare protein and peptide samples can interfere with analysis by ELISA, isoelectric focusing and mass spectrometry (MS). Removing detergents from peptide samples is especially challenging and critical for MS analysis because even low concentrations of detergents will contaminate instruments and interfere with column binding, elution and peptide ionization. The HiPPR Detergent Removal Resin is ideal for rapid detergent removal from tryptic digests to improve the results of LC-MS/MS and MALDI-MS analysis as to help maintain column and instrument performance over time.

数据：

Protocol summary for HiPPR Detergent Removal Spin Columns

HiPPR Detergent Removal Resin improves LC-MS/MS analysis of enzymatically digested BSABSA (100µg/mL) tryptic digests were prepared without detergent, in the presence of 0.5% Triton™ X-100 or spiked with 0.5% SDS following enzymatic digestion. Samples (0.1mL) containing detergent were processed with the HiPPR Detergent Removal Resin and compared to unprocessed or detergent-free samples by LC-MS/MS. Results demonstrate that detergents removal is effective and produces results similar to those observed for samples containing no detergent.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45640.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

HiPPR™ Detergent Removal Spin Columns, 0.1 mL,HiPPR™ Detergent Removal Spin Columns, 0.1 mL/HiPPR™洗涤剂去除离心柱

相关推荐