Mouse IgG2a kappa Isotype Control (eBM2a), PE-Cyanine7

货号：25-4724-81

规格：50μg

价格：1267

产品类型：小鼠同型对照

品牌：Thermo Fisher

物种：其它

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：PE-Cy7

类型:		同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

产品详细信息Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This eBM2a antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBM2a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

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参考文献：

1.PloS oneDengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptors."25-4724 was used in Flow cytometry/Cell sorting to examine the mechanisms of antibody dependent enhancement of Zika virus infection in primary human cells."AuthorsLi M,Zhao L,Zhang C,Wang X,Hong W,Sun J,Liu R,Yu L,Wang J,Zhang F,Jin X2.Cell communication and signaling : CCSThe CXCR4 antagonist plerixafor (AMD3100) promotes proliferation of Ewing sarcoma cell lines in vitro and activates receptor tyrosine kinase signaling."25-4724 was used in Flow cytometry/Cell sorting to investigate the CXCR4 receptor antagonist, plerixafor, for anti-cancer activity in Ewing sarcoma."AuthorsBerning P,Schaefer C,Clemens D,Korsching E,Dirksen U,Potratz J

技术参数

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