IL-1 alpha Human ELISA Kit ELISA试剂盒,IL-1 alpha Human ELISA Kit/人IL-1 α ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

IL-1 alpha Human ELISA Kit ELISA试剂盒,IL-1 alpha Human ELISA Kit/人IL-1 α ELISA试剂盒

2024-10-23

IL-1 alpha Human ELISA Kit/人IL-1 α ELISA试剂盒

货号：BMS243-2,BMS243-2TEN

规格：96 Tests,10X96 Tests

价格：1,34832

产品类型：Platinum ELISA

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	1.1 pg/mL
检测范围	1.6-100 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL 尿液 50 μL
操作时间	1 hr 20 min
测试时间	3 hr 10 min
Homogenous (no wash)	No
批间变异系数（CV）	10%
批内变异系数（CV）	5.4%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	10 x 96 tests
组份	- 预包被96孔板 - 标准品 - 样品稀释液 - 测定稀释浓缩液 - 生物素化检测抗体 - SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖
储存温度	2-8℃
Protein name	IL-1 alpha
Protein aliases	Hematopoietin-1, IL-1 alpha, interleukin 1, alpha, Interleukin-1 alpha, preinterleukin 1 alpha, pro-interleukin-1-alpha
物种（Tested）	人
试剂盒类型	Sandwich ELISA Kit
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	IL-1A, IL1, IL1-ALPHA, IL1A, IL1F1
Gene ID	(Human) 3552
UniProt ID	(Human) P01583
Gene symbol	IL1A

The Human Interleukin-1alpha (Hu IL-1α) ELISA quantitates Hu IL-IL-1α in human serum, plasma, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1α.人白介素-1α（人IL-1α）ELISA定量测定人血清，血浆，尿液或细胞培养基中的人IL-IL-1α。该检测试剂盒将专门识别天然和重组的人IL-1α。Principle of the method 方法原理The Human IL-1α solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IL-1α固相夹心ELISA（酶联免疫吸附测定）旨在测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每批生产都经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IL-1 alpha (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 alpha is coded by the IL1A gene and signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 beta. IL1A is located on the q arm on chromosome 2 at position 13. IL-1 alpha plays an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. IL-1 alpha activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. Further, IL-1 alpha is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury and induces apoptosis. The IL1A gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. IL1 alpha regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-1 alpha can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-1 alpha are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.IL-1 alpha（白介素-1 alpha）是由单核细胞，巨噬细胞和树突状细胞表达的促炎细胞因子。 IL-1 alpha由IL1A基因编码，并通过两个受体IL-1RI和IL-1RII发出信号，这两个受体均与IL-1 beta共享。 IL1A位于2号染色体的13位q臂上。IL-1alpha通过触发靶细胞中其他促炎细胞因子的产生并启动急性期反应，在先天宿主防御中发挥重要作用。 IL-1受体拮抗剂（IL-1RA）可以调节IL-1α的活性，IL-1RA是许多细胞类型产生的蛋白质，通过竞争性抑制作用阻断受体结合。 IL-1α是参与多种免疫反应，炎症过程和造血作用的多效细胞因子。此外，IL-1α由单核细胞和巨噬细胞作为原蛋白产生，其被蛋白水解处理并响应细胞损伤而释放并诱导凋亡。 IL1A基因和其他8个白介素1家族基因在2号染色体上形成细胞因子基因簇。IL1α调节NF-κB和促分裂原活化蛋白激酶的活性。 IL-1α可以刺激IL6和环氧合酶2（PTGS2 / COX-2）的表达，并增强一氧化氮（NO）的产生。高水平的IL-1α与几种慢性炎症性疾病有关，包括类风湿性关节炎，牛皮癣和多发性硬化症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

数据：

IL-1 alpha Human ELISA KitRepresentative standard curve for Human IL-1 alpha ELISA

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-45710.htm

特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系删除，本平台不提供信息存储服务。

Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.

产品询价需求提交

IL-1 alpha Human ELISA Kit ELISA试剂盒,IL-1 alpha Human ELISA Kit/人IL-1 α ELISA试剂盒

相关推荐