Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803)

货号：14-9008-82

规格：100μg

价格：3496

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：KIKSI0803

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent (Flow)

产品详细信息Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated KIKSI0803 is recommended for use in intracellular flow cytometry.).Applications Tested: This KIKSI0803 antibody has been tested by immunoblotting of stimulated normal human peripheral blood cells. This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

数据

Phospho-STAT1 (Tyr701) Antibody (14-9008-82) in WBTOP: Immunoblotting of reduced lysates from normal human peripheral blood cells left unstimulated (lane 1) or stimulated with IFN gamma (lane 2) using 5 µg/mL of Anti-Human phospho-STAT1 (Y701) Purified.BOTTOM: Total STAT1 was used as a loading control.Bands were visualized with Anti-Mouse IgG HRP.

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参考文献：

1.Journal of inflammation (London, England)Inhibition of pSTAT1 by tofacitinib accounts for the early improvement of experimental chronic synovitis."14-9008 was used in Western Blotting to study the early effects drawn by JAK inhibitors on intra-joint JAK/STAT-dependent signalling."AuthorsPérez-Baos S,Gratal P,Barrasa JI,Lamuedra A,Sánchez-Pernaute O,Herrero-Beaumont G,Largo R

技术参数

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