Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), PE-Cyanine7
货号:25-9008-41,25-9008-42
规格:25 Tests,100 Tests
价格:1312,3294
产品类型:流式抗体
品牌:eBioscience
物种:人
宿主:小鼠
抗体亚型:IgG1, kappa
克隆号:KIKSI0803
荧光染料:PE-Cy7
类型: | 单抗 | 同型对照: | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7 |
浓度: | 5 µL/Test | 用法: | 5 µL (0.125 µg)/test(Flow) |
产品详细信息Description: This KIKSI0803 monoclonal antibody recognizes signal transducer and activator of transcription 1 (STAT1) when phosphorylated on tyrosine 701. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. Phosphorylation of STAT1 on tyrosine 701 by JAK1 and JAK2 is essential for STAT1 dimer formation, nuclear translocation, and DNA binding activity. In response to IFN gamma stimulation, STAT1 homodimerizes or forms heterodimers with STAT3 that can bind to GAS (IFN gamma-activated sequence) promoter elements. In response to either IFN alpha or IFN beta stimulation, STAT1 forms a heterodimer with STAT2 that can bind ISRE (IFN-stimulated response element) promoter elements.Specificity of this KIKSI0803 clone was determined by ELISA, flow cytometry, and western blotting.Applications Reported: This KIKSI0803 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This KIKSI0803 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using Protocol C: Two-step protocol for Fixation/Methanol. Protocol A: Two-step protocol for intracellular (cytoplasmic) proteins and Protocol B: One-step protocol for intracellular (nuclear) proteins cannot be used. All Protocols can be found in “Staining Intracellular Antigens for Flow Cytometry" located atwww.thermofisher.com/flowprotocols. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser靶标信息STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
数据 |
Phospho-STAT1 (Tyr701) Antibody (25-9008-42) in FlowNormal human peripheral blood cells were unstimulated (orange histogram) or stimulated for 15 minutes with Human IFN gamma Recombinant Protein (Product # BMS303) (purple histogram). Cells were then stained intracellularly, using the IC Fixation Buffer (Product # 00-8222-49) and Fixation/Methanol protocol, with Phospho-STAT1 (Tyr701) Monoclonal Antibody, PE-Cyanine7. CD14+ CD4-low cells in the monocyte gate were used for analysis. |
推荐产品: |
二抗荧光二抗免疫组化一抗标签抗体 |
©
bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://gibcohyclone.cn/thread-45773.htm
特别声明:以上内容(如有图片亦包括在内)来源于授权厂家或网络,如有侵权,请联系删除,本平台不提供信息存储服务。
Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.