Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), FITC

货号：11-9010-41,11-9010-42

规格：25 Tests,100 Tests

价格：0,3202

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：SRBCZX

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test(Flow)

产品详细信息Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity.Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.Applications Reported: This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated U937 cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

数据

Phospho-STAT5 (Tyr694) Antibody (11-9010-42) in FlowIntracellular staining of untreated (orange histogram) or 15-minute Human GM-CSF Recombinant Protein-treated (purple histogram,Product # 14-8339-62) U937 cells with Anti-Human/Mouse phospho-STAT5 (Y694) FITC. Total viable cells were used for analysis.

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参考文献：

1.eLifeReceptor-mediated dimerization of JAK2 FERM domains is required for JAK2 activation."Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 11-9010-42) in Flow Cytometry"2.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchInactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation."11-9010 was used in Flow cytometry/Cell sorting to demonstrate a novel role for mechanistic target of rapamycin complex 1 in the regulation of bone marrow environments that support B-cell differentiation via regulating IL-7 expression."AuthorsWang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

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