Nitric Oxide Assay Kit/一氧化氮测定试剂盒

货号：EMSNO

规格：2 x 96 tests

价格：6161

产品类型：多因子检测

品牌：Thermo Fisher

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

The Nitric Oxide Assay Kit determines nitric oxide composition through measurement of nitrate (NO3) and nitrite (NO2) levels in urine, saliva, plasma, serum, and other biological fluids.Characteristics of the Nitric Oxide Assay Kit:Target:nitric oxide and relative levels of nitrite and nitrateFormat:colorimetric stain (azo dye) for clear 96-well platesMeasurement:absorbance at 540 nm (±20 nm)Assay range:0 to 100 μMSensitivity:0.222 μM nitrite; 0.625 μM nitratePrecision:dependent upon several factors (generally, intra- and inter-assay CV ≤ 5%)Sample types:urine, saliva, plasma, serum, and other biological fluidsTotal assay time:15 minutes (nitrite assay); 45 minutes (nitrate assay)Sample size:50 µLThe Nitric Oxide Assay Kit is for quantitative determination of nitrite and nitrate in biological fluids. The kit uses the enzyme nitrate reductase to convert nitrate to nitrite. Nitrite is then detected as a colored azo dye product of the Griess reaction that absorbs visible light at 540 nm. The interaction of nitric oxide in a system is measured by the determination of both nitrate and nitrite concentrations in the sample. The relative levels of nitrite and nitrate can vary substantially, therefore the most accurate determination of total NO production requires measurement of both nitrate and nitrite.See manual for sample preparation guidelines, sample-specific tips, and calculations.Includes:Griess reagents, nitrite and nitrate standards, nitrate reductase, NADH, and diluents.Two microplates are necessary to perform this assay. One plate is used to measure the amount of nitrite in each sample. For the second plate, all of the nitrate in each sample is converted to nitrite using nitrate reductase enzyme and the “total” amount of nitrite (pre-existing nitrite plus nitrate that was converted) is measured. The nitrite measurements from plate 1 are subtracted from the total nitrite measurements in plate 2 in order to calculate nitrate concentrations. This kit is specifically designed for researchers concerned with the relative levels of nitrate and nitrite in each sample.

技术参数

组成成分 • Reagent diluent • Nitrate reductase diluent • Nitrate reductase • NADH • Nitrite standard • Nitrate standard • Griess reagents • Plates and plate sealer

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