Mouse IFN beta ProQuantum Immunoassay Kit,Mouse IFN beta ProQuantum Immunoassay Kit/小鼠IFN β ProQuantum免疫测定试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse IFN beta ProQuantum Immunoassay Kit,Mouse IFN beta ProQuantum Immunoassay Kit/小鼠IFN β ProQuantum免疫测定试剂盒

2024-10-23

Mouse IFN beta ProQuantum Immunoassay Kit/小鼠IFN β ProQuantum免疫测定试剂盒

货号：A47435,A474355

规格：96 Tests,5X96 Tests

价格：7550,33970

产品类型：Proquantum ELISA

品牌：Thermo Fisher

物种：小鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<0.3 pg/mL
检测范围	0.32-5,000 pg/mL
样本类型/体积	血浆 2-5 μL 血清 2-5 μL 上清液 2-5 μL
操作时间	30 min
测试时间	2 hr
Homogenous (no wash)	No
规格	5 X 96 Tests
组份	- 分析稀释缓冲液 - 抗体结合物 A - 抗体结合物 B - 抗体结合稀释缓冲液 - 标准品 - 预混料 - 连接酶
储存温度	-20℃
运输条件	干冰
Protein name	IFN beta
Protein aliases	IFN-beta, Interferon beta
物种（Tested）	小鼠
试剂盒类型	Proximity-based Immunoassay
标记（染料）	Unconjugated
Gene ID	(Mouse) 15977
Gene aliases	Ifb, IFN-beta, IFNB, Ifnb1
Gene symbol	Ifnb1
UniProt ID	(Mouse) P01575

The ProQuantum Mouse IFN beta Immunoassay Kit is designed to provide quantitative measurements of mouse IFN beta in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.ProQuantum小鼠IFNβ免疫测定试剂盒旨在提供小样品量中小鼠IFNβ的定量测量。该分析利用基于邻近的扩增技术，将高亲和力抗体-抗原结合的分析物特异性与实时PCR的信号检测和扩增功能结合在一起，从而实现了一个简单而强大的下一代蛋白质定量平台。用户友好的工作流程与直观的分析软件相结合，可在2小时内完成从样品到答案的分析。ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1).ProQuantum免疫测定利用一对配对的靶标特异性抗体，每种抗体均与DNA寡核苷酸偶联。在抗体-分析物结合过程中，两个DNA寡核苷酸紧密靠近，这允许两条链连接并随后产生模板链进行扩增。该平台利用了Applied Biosystems TaqMan实时PCR技术的灵敏度和较大的动态范围（图1）。The assay workflow is fast and easy—2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum software (cloud-based version athttps://apps.thermofisher.com/apps/proquantum). Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, apply a 5PL weighted algorithm, and obtain robust statistical group-wise comparisons.测定工作流程快速简便— 2小时内完成2个步骤。每个套件中总共有7个组件（图2）。首先，混合抗体-缀合物，稀释样品，并在工作板上创建标准曲线。然后，使用多通道移液器，将抗体结合物和样品（或标准品）添加到PCR板的孔中，并孵育1小时。合并预混液和连接酶，并添加到PCR板的孔中，然后在任何qPCR仪器上运行该板。运行完成后，将结果文件导入ProQuantum软件（基于云的版本，网址为https://apps.thermofisher.com/apps/proquantum）。使用此软件，可以轻松分析数据以获得蛋白质浓度值。该软件可让您设置标准曲线，设计板布局，应用5PL加权算法并获得可靠的逐组统计比较。Target information 靶标信息Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.I型干扰素（IFN-α/β）主要是由于天然干扰素产生细胞（IPC）对病毒感染的反应而产生，是宿主免疫反应的一部分。干扰素也可以抑制肿瘤的发展。 IFN-β结合导致酪氨酸激酶Jak1和Tyk2活化，转录因子STAT家族成员的磷酸化以及免疫应答基因的转录和表达。最近，人们发现了Toll样受体（TLR）家族的几个成员可以刺激IFN-β的产生。 IFN-β目前在临床上用于治疗肿瘤，感染和多发性硬化症。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

特点：

- 高灵敏度 - 更高的灵敏度检测低水平的蛋白质- 宽动态范围- 样品消耗少 - 使用2–5 µL样品- 快速，轻松的工作流程 - 从样品到取样仅需2个小时，无需清洗- 无需购买专有仪器 - 可在任何实时PCR仪器上运行

数据：

Mouse IFN β ProQuantum KitHow ProQuantum immunoassays work. (A) Antibody-antigen binding: Antibodies bind to two separate epitopes on the antigen (during a 1-hr incubation), which brings the two conjugated oligonucleotides into close proximity. (B) Ligation and amplification of signal (in a qPCR instrument): DNA ligase and a third splint oligonucleotide connector are added to ligate the two ends of the conjugated oligonucleotides, creating a 100-base DNA template. Once the ligase is inactivated at 95°C, the sample is amplified through 40 cycles of annealing and extension. The amount of DNA produced is measured after each amplification cycle via fluorescent dyes, which exhibit fluorescence that is directly proportional to the number of PCR product molecules (or amplicons) generated. This graph shows the fluorescence vs. cycle number curves for four different starting protein concentrations. The dashed line represents the fluorescence threshold. The cycle number required to reach the fluorescence threshold (threshold cycle or Ct) is plotted vs. protein concentration to create a standard curve.小鼠IFN β ProQuantum试剂盒ProQuantum免疫测定如何工作。（A）抗体-抗原结合：抗体与抗原上的两个独立表位结合（在1小时的孵育过程中），这使两个结合的寡核苷酸紧密相邻。（B）信号的连接和扩增（在qPCR仪器中）：添加DNA连接酶和第三个夹板寡核苷酸连接物以连接缀合的寡核苷酸的两端，从而产生100个碱基的DNA模板。连接酶在95°C失活后，样品会通过40个退火和延伸循环进行扩增。在每个扩增循环后，通过荧光染料测量产生的DNA量，该荧光染料显示的荧光与产生的PCR产物分子（或扩增子）的数量成正比。该图显示了四种不同起始蛋白质浓度的荧光与循环数曲线。虚线表示荧光阈值。将达到荧光阈值所需的循环数（阈值循环或Ct）对蛋白质浓度作图，以创建标准曲线。

MouseIFN β ProQuantum KitProQuantum kit contents. Components are color coded for ease of use.

技术参数

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Mouse IFN beta ProQuantum Immunoassay Kit,Mouse IFN beta ProQuantum Immunoassay Kit/小鼠IFN β ProQuantum免疫测定试剂盒

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