CD3e Monoclonal Antibody (145-2C11), APC, eBioscience™

货号：17-0031-63,17-0031-81,17-0031-82,17-0031-83

规格：10 µg,50 µg,100 µg,200 µg

价格：422,1567,1197,3603

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：145-2C11

荧光染料：APC

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™
浓度：	0.2 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3e Antibody (17-0031-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (left) or 0.125 µg of Anti-Mouse CD3e APC (right). Total viable cells were used for analysis.

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参考文献：

1. Molecular pharmaceuticsEffect of the poly(ethylene glycol) (PEG) density on the access and uptake of particles by antigen-presenting cells (APCs) after subcutaneous administration."17-0031 was used in Flow cytometry/Cell sorting to study the effect of PEGylation on the lymphatic trafficking and accumulation of particles in draining lymph nodes."AuthorsZhan X,Tran KK,Shen HYear2012SpeciesMouse 2. Journal of the American Heart AssociationDeleterious effect of the IL-23/IL-17A axis and γδT cells on left ventricular remodeling after myocardial infarction."17-0031 was used in Flow cytometry/Cell sorting to explore potential immune pathways involved in left ventricular remodelling as possible preventative therapeutic options."AuthorsYan X,Shichita T,Katsumata Y,Matsuhashi T,Ito H,Ito K,Anzai A,Endo J,Tamura Y,Kimura K,Fujita J,Shinmura K,Shen W,Yoshimura A,Fukuda K,Sano MYear2012SpeciesMouse 3. Infection and immunityHost response signature to Staphylococcus aureus alpha-hemolysin implicates pulmonary Th17 response."17-0031 was used in Flow cytometry/Cell sorting to evaluate the transcriptomic response to Hla-secreting staphylococci infection in humans."AuthorsFrank KM,Zhou T,Moreno-Vinasco L,Hollett B,Garcia JG,Bubeck Wardenburg JYear2012SpeciesMouse 4. Journal of lipid researchCharacterization of an arachidonic acid-deficient (Fads1 knockout) mouse model."17-0031 was used in Flow cytometry/Cell sorting to determine the utility of antagonising tissue arachidonic acid (AA) levels as a novel approach to suppress AA-derived eicosanoids."AuthorsFan YY,Monk JM,Hou TY,Callway E,Vincent L,Weeks B,Yang P,Chapkin RSYear2012SpeciesMouse 5. Journal of immunology (Baltimore, Md. : 1950)The Thai phase III trial (RV144) vaccine regimen induces T cell responses that preferentially target epitopes within the V2 region of HIV-1 envelope."17-0031 was used in Flow cytometry/Cell sorting to reveal T cell response of HIV-1 vaccine recipients was directed to a particular region of viral envelope."Authorsde Souza MS,Ratto-Kim S,Chuenarom W,Schuetz A,Chantakulkij S,Nuntapinit B,Valencia-Micolta A,Thelian D,Nitayaphan S,Pitisuttithum P,Paris RM,Kaewkungwal J,Michael NL,Rerks-Ngarm S,Mathieson B,Marovich M,Currier JR,Kim JH

技术参数

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