OCT4 Recombinant Polyclonal Antibody (3HCLC)/多克隆抗体

货号：710788

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to Human POU5F1 (aa 280-291)

物种：人

宿主：兔

抗体亚型：IgG

克隆号：3HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg x 10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Goat, Cat and HorseRecombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

数据：

OCT4 Antibody (710788) in IFFor immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of endogenous OCT4 using Anti-OCT4 Recombinant Rabbit Polyclonal Antibody (Product # 710788, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of OCT4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of OCT4. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. OCT4 Antibody (710788) in WBKnockdown of OCT4 was achieved by transfecting NTERA-2 cells with OCT4 specific siRNA (Silencer® select Cat # s10871, Product # s10873). Western blot analysis (Fig a) was performed using Whole cell extract from the OCT4 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-OCT4 Recombinant Rabbit Polyclonal Antibody (Product # 710788, 2 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:5000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to OCT4. OCT4 Antibody (710788)Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NTERA-2 cells were transfected with OCT4 siRNA and loss of signal was observed in Western blot using Anti-OCT4 Recombinant Rabbit Polyclonal Antibody (Product # 710788).

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