Histone Macro-H2A.1 Recombinant Rabbit Monoclonal Antibody (RM248)/单克隆抗体

货号：MA5-24696

规格：100 µg

价格：4130

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to the C-terminus of human H

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：RM248

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；0.2-1 µg/mL(ELISA)；1-2 µg/mL(ICC)；1-2 µg/mL(IF)；1 µg/mL(WB)

产品详细信息This antibody reacts to the Core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross reactivity with other histone proteins.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

Histone Macro-H2A.1 Antibody (MA5-24696) in IFImmunofluorescence analysis of Core Histone Macro-H2A.1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Core Histone Macro-H2A.1 (Product # MA5-24696) at 5 microgram/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Histone Macro-H2A.1 Antibody (MA5-24696) in WBWestern blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), U-2 OS (Lane 3), Hep G2 (Lane 4) and NIH/3T3 (Lane 5). The blot was probed with Anti-Core Histone Macro-H2A.1 Monoclonal Antibody (Product # MA5-24696, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 39 kDa band corresponding to Core Histone Macro-H2A.1 was observed across the cell lines tested. Histone Macro-H2A.1 Antibody (MA5-24696) in ChIPEnrichment of endogenous Methyl-Histone H3 (Lys9) protein at specific gene loci using Anti-Methyl-Histone H3 (Lys9) Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Methyl-Histone H3 (Lys9) Polyclonal Antibody (Product # PA5-11183, 3 µg) on sheared chromatin from 2 million HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the MYOD gene used as positive and c-Fos, GAPDH, SAT2 satellite repeats used as negative target genes/binding sites. . Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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