c-Jun Recombinant Rabbit Monoclonal Antibody (2H4L1)/c-Jun重组兔单克隆抗体（2H4L1）

货号：702170

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to Human JUN (aa 21-36, 211

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：2H4L1

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg x 10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Bovine and PigRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animalorigin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

数据：

c-Jun Antibody (702170) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of endogenous c-jun using Anti-c-jun Recombinant Rabbit Monoclonal Antibody (Product # 702170, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of c-jun protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of c-jun. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. c-Jun Antibody (702170) in WBWestern blot analysis was performed on nuclear extracts (30 µg lysate) of NIH/3T3 (Lane 1), HEK-293 (Lane 2), PC-3 (Lane 3), U-87 MG (Lane 4) and U-2 OS (Lane 5). The blots were probed with Anti-c-jun Recombinant Rabbit Monoclonal Antibody (Product # 702170, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 36 kDa band corresponding to c-jun was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot®2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). c-Jun Antibody (702170) in ChIPEnrichment of endogenous c-Jun protein at specific gene loci using Anti-c-Jun Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-c-Jun Recombinant Rabbit Monoclonal Antibody (Product # 702170, 5 µg) on sheared chromatin from 2 million U-937 cells treated with 250 ng/mL anisomycin for 30 minutes using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active JAG1 region used as positive control target genes, and the region of the inactive MYOD, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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