CREB Monoclonal Antibody (LB9)/CREB单克隆抗体（LB9）

货号：MA1-083

规格：100 µg

价格：5468

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant human CREB-A protein.

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：LB9

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；0.1-1.0 µg/mL(ELISA)；5 µg/mL(ICC)；5 µg/mL(IF)；1:200(IHC)；2 µg(IP)；1:500(WB)

产品详细信息MA1-083 detects the CREB in human, monkey, rat and mouse cells.MA1-083 has been successfully used in immunofluorescence, Western blot, immunoprecipitation, immunohistochemistry, and ELISA procedures. By Western blot, this antibody detects a ~43 kDa protein.The MA1-083 immunogen is human recombinant CREB-A protein.靶标信息CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer's Disease, histiocytoma and soft tissue melanoma.

数据：

CREB Antibody (MA1-083) in IFImmunofluorescence analysis of CREB was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CREB Monoclonal Antibody (Product # MA1-083) at 5µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. CREB Antibody (MA1-083) in IFImmunofluorescent analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing CREB (Product # MA1-083), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification. CREB Antibody (MA1-083) in IHCImmunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CREB (Product # MA1-083) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

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参考文献：

1. Molecules (Basel, Switzerland)Chenodeoxycholic Acid Ameliorates AlCl3-Induced Alzheimer's Disease Neurotoxicity and Cognitive Deterioration via Enhanced Insulin Signaling in Rats.2. Translational psychiatryS-nitrosylation of E3 ubiquitin-protein ligase RNF213 alters non-canonical Wnt/Ca+2 signaling in the P301S mouse model of tauopathy.3. Journal of molecular and cellular cardiologyMitochondrial remodeling in mice with cardiomyocyte-specific lipid overload.

技术参数

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