DLX2 Recombinant Rabbit Monoclonal Antibody (1H13L11)/DLX2重组兔单克隆抗体（1H13L11）

货号：702009

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to human DLX2 [1) aa127-aa1

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：1H13L11

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；5 µg/mL(ICC)；5 µg/mL(IF)；2.5 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Pig, Bovine, MouseRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 2.

数据：

DLX2 Antibody (702009) in IFFor immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of endogenous DLX2 using Anti- DLX2 Recombinant Rabbit Monoclonal Antibody (Product # 702009, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DLX2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of DLX2. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. DLX2 Antibody (702009) in WBWestern blot analysis was performed on Modified Whole cell extracts (1% SDS) (30 µg lysate) of SH-SY5Y (Lane 1) and Neuro-2a (Lane 2). The blots were probed with Anti-DLX2 Recombinant Rabbit Monoclonal Antibody (Product # 702009, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 34 kDa band corresponding to DLX2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). DLX2 Antibody (702009) in ChIPEnrichment of endogenous DLX2 protein at specific gene loci using Anti-DLX2 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-DLX2 Recombinant Rabbit Monoclonal Antibody (Product # 702009, 5 µg) on sheared chromatin from 2 million NTERA-2 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the OLIG2, NKX2.2, MYT1 used as positive control target genes, and SAT2 satellite repeat used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. Cell reportsAn Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons.

技术参数

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