E2F3 Monoclonal Antibody (3E2F04 (PG37))/E2F3单克隆抗体（3E2F04（PG37））

货号：MA5-11319

规格：500 µL

价格：4642

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant human E2F-3 protein

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：3E2F04 (PG37)

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.2 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；1mg/mL(GS)；Assay Dependent(IF)；2-3 µg/mL(WB)

产品详细信息MA5-11319 targets E2F-3 Transcription Factor in GS and IF applications and shows reactivity with Human samples.The MA5-11319 immunogen is recombinant human E2F-3 protein.靶标信息The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

数据：

E2F3 Antibody (MA5-11319) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), MDA-MB-231 (Lane 4), PC-3 (lane 5). The blots were probed with Anti-E2F3 Transcription Factor Mouse Monoclonal Antibody (Product # MA5-11319, 2 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). Two bands 37 and 49 kDa band corresponding to E2F3 Transcription Factor was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). E2F3 Antibody (MA5-11319) in ChIPEnrichment of endogenous E2F3 Protein at specific gene loci using Anti-E2F3 Transcription Factor Mouse Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-E2F3 Transcription Factor Mouse Monoclonal Antibody (Product # MA5-11319, 5 µg) on sheared chromatin from 2 million K562 cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of active E2F3, ATATD2, RAD51 gene, used as positive control target, and the SAT2, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. PloS oneIntegrated genomic and gene expression profiling identifies two major genomic circuits in urothelial carcinoma.2. Journal of molecular and cellular cardiologyAnti-apoptotic function of the E2F transcription factor 4 (E2F4)/p130, a member of retinoblastoma gene family in cardiac myocytes.3. OncogeneInactivation of the Rb pathway and overexpression of both isoforms of E2F3 are obligate events in bladder tumours with 6p22 amplification.

技术参数

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