ROR gamma (t) Monoclonal Antibody (AFKJS-9), APC, eBioscience™/ROR gamma (t) 单抗 (AFKJS-9), APC, eBioscience™

货号：17-6988-82,17-6988-80

规格：100 ug,25 ug

价格：4666,3274

产品类型：流式抗体

品牌：eBioscience

物种：大鼠

宿主：驴

抗体亚型：IgG2a, kappa

克隆号：AFKJS-9

荧光染料：APC

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™
浓度：	0.2 mg/mL	用法：	1 µg/test(Flow)

产品详细信息Description: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma (t), which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymocytes, Th17 cells of the periphery and lymphoid tissue inducer (LTi) cells of lymphoid organs.The ROR gamma (t) isoform differs from ROR gamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the ROR gamma and ROR gamma (t) isoforms.Applications Reported: This AFKJS-9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This AFKJS-9 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This antibody can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion inimmature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

ROR gamma (t) Antibody (17-6988-82) in FlowSurface staining of mouse thymocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and Anti-Mouse CD8a PE (Product # 12-0081-82) (left), followed by intracellular staining with 0.5 µg of Anti-Human/Mouse ROR gamma (t) APC (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4+CD8+ (purple histogram) or CD4+CD8- (blue histogram) gate were used for analysis.

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参考文献：

1.Wellcome open researchCharacterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.2.F1000ResearchMyelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.3.Cancer cellTargeting TMEM176B Enhances Antitumor Immunity and Augments the Efficacy of Immune Checkpoint Blockers by Unleashing Inflammasome Activation.4.PloS oneLack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis.5.CellSystemic Human ILC Precursors Provide a Substrate for Tissue ILC Differentiation.

技术参数

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