T-bet Monoclonal Antibody (eBio4B10 (4B10)), PE, eBioscience™/T-bet 单克隆抗体 (eBio4B10 (4B10)), PE, eBioscience™

货号：12-5825-82,12-5825-80

规格：100 ug ,25ug

价格：4101,2055

产品类型：流式抗体

品牌：eBioscience

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBio4B10 (4B10)

荧光染料：PE

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eBio4B10 monoclonal antibody reacts with mouse, rhesus moneky and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio4B10 antibody has been tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This antibody can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.For intracellular staining with the eBio4B10 monoclonal antibody, it is recommended to use the eBioscience Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for intracellular (nuclear) proteins).Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

T-bet Antibody (12-5825-82) in FlowIntracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0086-42) and 0.25 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.25 µg of Anti-Human/Mouse T-bet PE (right) using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Frontiers in immunologyInducible Costimulator Expressing T Cells Promote Parasitic Growth During Blood StagePlasmodium bergheiANKA Infection.2.Nature communicationsStabilization of cytokine mRNAs in iNKT cells requires the serine-threoninekinase IRE1alpha.3.Nature communicationsRegulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.4.CellInitiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.5.Nature communicationsStabilization of cytokine mRNAs in iNKT cells requires the serine-threoninekinase IRE1alpha.

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