CD45RB Monoclonal Antibody (C363.16A), PE, eBioscience™/CD45RB单抗 (C363.16A), PE, eBioscience™

货号：12-0455-82,12-0455-83

规格：100 ug,200ug

价格：1011,2019

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：C363.16A

荧光染料：PE

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
浓度：	0.2mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45. This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis.Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD45RB Antibody (12-0455-82) in FlowStaining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.125 µg of Anti-Mouse CD45RB PE (filled histogram). Total viable cells were used for analysis.

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参考文献：

1.Cell death and diseaseCD69 enhances immunosuppressive function of regulatory T-cells and attenuates colitis by prompting IL-10 production.2.Journal of immunology (Baltimore, Md. : 1950)Loss of the Protein Tyrosine Phosphatase PTPN22 Reduces Mannan-Induced Autoimmune Arthritis in SKG Mice.3.Mucosal immunologyFoxp3(+) T cells expressing RORγt represent a stable regulatory T-cell effector lineage with enhanced suppressive capacity during intestinal inflammation.4.Mucosal immunologyβ7-Integrin exacerbates experimental DSS-induced colitis in mice by directing inflammatory monocytes into the colon.5.PloS oneCalcitonin gene-related peptide regulates type IV hypersensitivity through dendritic cell functions.

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