HMGN1 Polyclonal Antibody/HMGN1多克隆抗体

货号：720387

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to human HMGN1 [1) aa42-aa6

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；5 µg/mL(ICC)；5 µg/mL(IF)；2 µg/mL(WB)

产品详细信息This antibody predicted to react with Monkey, Bovine, Sheep靶标信息The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates. HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of the higher order chromatin structure thereby facilitating access to the underlying DNA sequence. Clustering of architectural elements, such as HMG proteins and linker histone subtypes into distinct domains, may lead to structural and functional heterogeneity along the chromatin fiber. In addition, HMG-14 and HMG-17 have been identified as constitutive components of mouse oocyte and embryonic chromatin that establish a link between the structure of embryonic chromatin and the normal progression of embryonic development.

数据：

HMGN1 Antibody (720387) in ICCFor immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous HMGN1 using Anti- HMGN1 Rabbit Polyclonal Antibody (Product # 720387, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HMGN1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of HMGN1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. HMGN1 Antibody (720387) in ChIPEnrichment of endogenous HMGN1 protein at specific gene loci using Anti-HMGN1 Rabbit Polyclonal Antibody: Chromatin Immunoprecipiation (ChIP) was performed using Anti-HMGN1 Rabbit Polyclonal Antibody (Product # 720387, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active MECP2, HBD2, DNase I Hypersensitive region used as positive control target genes, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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