KLF9 Recombinant Rabbit Monoclonal Antibody (5H16L7)/KLF9重组兔单克隆抗体（5H16L7）

货号：701888

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to Human KLF9 (aa 34-49, 85

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

克隆号：5H16L7

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2-3 µg/1x10^6 cells(ChIP)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Bovine, Pig and Monkey.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animalorigin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息KLF-9 is a transcription factor belonging to the Sp 1 C2H2 type zinc finger family. It binds to GC box elements located in the promoter. This binding of the encoded protein to a single GC box inhibits mRNA expression while binding to tandemly repeated GC box elements activates transcription. KLF-9 serves as a key transcriptional regulator for uterine endometrial cell proliferation, adhesion, and differentiation both during pregnancy and tumorigenesis.

数据：

KLF9 Antibody (701888) in WBWestern blot analysis was performed on extracts (30 µg of lysate) of SK-OV-3 (Lane 1), HEL (Lane 2), KARPAS 299 (Lane 3), HEK-293 (Lane 4), U-87 MG (Lane 5), Jurkat (Lane 6), Mouse Liver (Lane 7), Rat Liver (Lane 8). The blots were probed with KLF9 Recombinant Rabbit Monoclonal Antibody (Product # 701888, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 27 kDa band corresponding to KLF9 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). KLF9 Antibody (701888) in ChIPEnrichment of endogenous KLF9 protein at specific gene loci using Anti-KLF9 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-KLF9 Recombinant Rabbit Monoclonal Antibody (Product # 701888, 5 µg) on sheared chromatin from 2 million K562 cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active NOXA region used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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