Leptin Polyclonal Antibody/瘦素多克隆抗体

货号：PA1-051

规格：50 µL

价格：5468

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues Q(25)

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	2 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1:50 - 1:200(IHC (F))；1:200 - 1:1000(WB)

产品详细信息PA1-051 detects leptin from human, mouse, sheep and rat samples.PA1-051 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry experiments. By Western blot, this antibody detects an ~16 kDa protein representing leptin from 3T3-L1 cell extract. Immunohistochemical staining of leptin in sheep brain with PA1-051 results in staining of the ventromedial hypothalamus.The PA1-051 immunogen is a synthetic peptide corresponding to residues Q(25) K V Q DD T K T L I K T I V T R I N D(44) of mouse Leptin. This sequence is completely conserved between mouse and bovine.靶标信息Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.

数据：

Leptin Antibody (PA1-051) in IFImmunofluorescence analysis of Leptin was performed using 70% confluent log phase 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10070-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Leptin Rabbit Polyclonal Antibody (Product # PA1-051) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the undifferentiated cells that have lower level of Leptin expression. Panel f represents the no primary control. The images were captured at 60X magnification. Leptin Antibody (PA1-051)The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2008 -Published figure using Leptin polyclonal antibody (Product # PA1-051)

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参考文献：

1. Cellular signallingPalmitate-induced Endoplasmic Reticulum stress and subsequent C/EBPα Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain.2. Cellular signallingPalmitate-induced Endoplasmic Reticulum stress and subsequent C/EBPα Homologous Protein activation attenuates leptin and Insulin-like growth factor 1 expression in the brain.3. The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyLeptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.4. The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyLeptin and the obesity receptor (OB-R) in the small intestine and colon: a colocalization study.5. BMC cell biologyPressure mediated hypertrophy and mechanical stretch up-regulate expression of the long form of leptin receptor (ob-Rb) in rat cardiac myocytes.

技术参数

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