SUZ12 Recombinant Rabbit Monoclonal Antibody (7H26L21)/SUZ12重组兔单克隆抗体（7H26L21）

货号：702490

规格：100 µg

价格：4553

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Protein corresponding to human SUZ12 [aa545-aa725]

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：7H26L21

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；0.5 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Bovine, Pig, MouseRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息SUZ12 (polycomb protein SUZ12) is a zinc finger gene and has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. SUZ12 is also a component of the PRC2/EED-EZH2 complex which methylates Lys-9 (H3K9me) and Lys-27 (H3K27me) of histone H3 leading to transcriptional repression of the affected target gene. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

数据：

SUZ12 Antibody (702490) in ICCFor immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of endogenous SUZ12 using Anti- SUZ12 Recombinant Rabbit Monoclonal Antibody (Product # 702490, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SUZ12 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of panels a, b and c clearly demonstrating nuclear localization of SUZ12. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. SUZ12 Antibody (702490) in ChIPEnrichment of endogenous SUZ12 protein at specific gene loci using Anti-SUZ12 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-SUZ12 Recombinant Rabbit Monoclonal Antibody (Product # 702490, 5 µg) on sheared chromatin from 2 million Ntera2 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the different loci of the active CCND2, HOXA1, HOXA2 promoter region used as positive control target genes, and the region of the inactive NANOG promoter, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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