SUZ12 Recombinant Polyclonal Antibody/SUZ12重组多克隆抗体

货号：711520

规格：100 µg

价格：4692

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Protein corresponding to human SUZ12 [aa545-aa725]

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Bovine, Pig, and Mouse.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息SUZ12 (polycomb protein SUZ12) is a zinc finger gene and has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. SUZ12 is also a component of the PRC2/EED-EZH2 complex which methylates Lys-9 (H3K9me) and Lys-27 (H3K27me) of histone H3 leading to transcriptional repression of the affected target gene. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.

数据：

SUZ12 Antibody (711520) in ICCFor immunofluorescence analysis, Ntera2 cells were fixed and permeabilized for detection of endogenous SUZ12 using Anti- SUZ12 Recombinant Rabbit Polyclonal Antibody (Product # 711520, 2µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SUZ12 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of SUZ12. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. SUZ12 Antibody (711520) in ChIPEnrichment of endogenous SUZ12 protein at specific gene loci using Anti-SUZ12 Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-SUZ12 Recombinant Rabbit Polyclonal Antibody (Product # 711520, 5 µg) on sheared chromatin from 2 million NTERA-2 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the different loci of the active CCND2, HOXA1, HOXA2 promoter region used as positive control target genes, and the region of the inactive NANOG promoter, SAT2 satellite repeat used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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