ZNF217 Polyclonal Antibody/ZNF217多克隆抗体

货号：720352

规格：100 µg

价格：4692

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptides corresponding to human ZNF217 [1) aa1029-

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；5 µg/mL(ICC)；5 µg/mL(IF)；2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Monkey, Pig, Rabbit靶标信息Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.

数据：

ZNF217 Antibody (720352) in ICCFor immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous ZNF217using Anti- ZNF217 Rabbit Polyclonal Antibody (Product # 720352, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ZNF217 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating Nuclear localization of ZNF217. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. ZNF217 Antibody (720352) in ChIPEnrichment of endogenous ZNF217 protein at specific gene loci using Anti-ZNF217 Rabbit Polyclonal Antibody: Chromatin Immunoprecipiation (ChIP) was performed using Anti-ZNF217 Rabbit Polyclonal Antibody (Product # 720352, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active P21, BRCA region used as positive control target genes, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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