Histone H2A.X Polyclonal Antibody/组蛋白H2A.X多克隆抗体

货号：PA1-41004

规格：100 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Mixture of synthetic peptides containing residues

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；1:1000(ELISA)；5 µg/mL(IHC (P))；1 µg/mL(WB)

产品详细信息Suggested positive control: Jurkat or PBMC.靶标信息Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

数据：

Histone H2A.X Antibody (PA1-41004) in WBWestern blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and COS-7 (Lane 4). The blot was probed with Anti-Histone H2A.X Polyclonal Antibody (Product # PA1-41004, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to H2A.X was observed across the cell lines tested. Histone H2A.X Antibody (PA1-41004) in ChIPEnrichment of endogenous Histone 1F0 protein at specific gene loci using Anti-Histone 1F0 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone 1F0 Polyclonal Antibody (Product # PA5-51466, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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参考文献：

1. Scientific reports82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

技术参数

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