Histone H2B Polyclonal Antibody/组蛋白H2B多克隆抗体

货号：PA1-41058

规格：100 µg

价格：5831

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to amino acids 111

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；10 µg/mL(IHC (P))；2 µg/mL(WB)

产品详细信息This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila.Suggested positive control: Jurkat, PBMC.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

数据：

Histone H2B Antibody (PA1-41058) in WBWestern blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MCF7 (Lane 3), COS-7 (Lane 4) and NIH/3T3 (Lane 5). The blot was probed with Anti-Histone H2B (Product # PA1-41058, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to HistoneH2B was observed across the cell lines tested. Histone H2B Antibody (PA1-41058) in ChIPEnrichment of endogenous Histone 1F0 protein at specific gene loci using Anti-Histone 1F0 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone 1F0 Polyclonal Antibody (Product # PA5-38570, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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