Apelin Receptor Recombinant Rabbit Monoclonal Antibody (5H5L9)

货号：702069

规格：100 µg

价格：4809

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-2 µg/mL (WB)；2 µg/mL(ICC/IF)

产品详细信息This antibody is predicted to react with Monkey, Rabbit, Horse and DogRecombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息APLNR (Apelin Receptor) is a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified.

数据

Apelin Receptor Antibody (702069) in WBWestern blot analysis was performed on membrane enriched extract/ tissue extract (30 µg lysate) of HepG2 (Lane 1), NTERA-2 (Lane 2), HEL.92.1.7 (Lane 3), K562 (Lane 4), and mouse liver (Lane 5). The blots were probed with Anti-APLNR Recombinant Rabbit Monoclonal Antibody (Product # 702069, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 50 kDa band corresponding to APLNR protein was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

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