Histone H3 Recombinant Rabbit Monoclonal Antibody (24HC2 LC12)/组蛋白H3重组兔单克隆抗体（24HC2 LC12）

货号：701517,701517-20UG

规格：100 µg,20 µg

价格：4692,1449

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：proprietary

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

克隆号：24HC2 LC12

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；Assay-Dependent(ELISA)；2-4 µg(ICC)；2-4 µg(IF)；1 µg/mL(WB)

产品详细信息This antibody is predicted to react with bovine, non-human primate, rat and ovine based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

Histone H3 Antibody (701517) in IFImmunofluorescent analysis of Histone H3 (C-terminal) was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Histone H3 (C-term) Recombinant Rabbit Monoclonal Antibody (Product # 701517) at 2 µg-4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization of Histone H3 (C-terminal). Panel e shows no primary antibody control. The images were captured at 20X magnification. Histone H3 Antibody (701517) in ChIPEnrichment of endogenous Histone-3 protein at specific gene loci using Anti-Histone-3 Recombinant Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone-3 Recombinant Rabbit Monoclonal Antibody (Product # 701517, 5 µg) on sheared chromatin from 2 million A549 cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoter of MYT-1 gene, SAT 2, Satellite alpha (SAT A) Repeat used as positive control target, and promoter of C-FOS, PABPC1 used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-46384.htm