H2AK5ac Recombinant Polyclonal Antibody/H2AK5ac重组多克隆抗体

货号：710830

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to Human HIST1H2AG (aa 2-10)

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	2-3 µg/1x10^6 cells(ChIP)；1 µg/mL(ELISA)；2µg/mL(ICC)；2 µg/mL(IF)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Rat, Pig, Dog and Cat.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H2AK5ac Antibody (710830) in IFFor immunofluorescence analysis HeLa cells were fixed and permeabilized for detection of H2AK5ac using H2AK5ac Recombinant Rabbit Polyclonal Antibody (Product # 710830, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Detection and localization of H2AK5ac (green) in the nucleus can be clearly observed in cells treated with suberoylanilide hydroxamic acid (SAHA, 0.5 uM, 18 h) as compared to untreated cells. Antibody specificity was demonstrated by competition with the H2AK5ac peptide, which results in loss of signal. No competition was observed with the non-. H2AK5ac Antibody (710830) in ChIPEnrichment of endogenous H2AK5ac protein at specific gene loci using Anti-Acetyl Histone H2A (Lys5) Recombinant Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H2AK5ac Recombinant Rabbit Polyclonal Antibody (Product # 710830, 5 µg) on sheared chromatin from 2 million Jurkat cells treated with 5mM sodium butyrate for 24 hours using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG (1 µg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active BActin, and cFOS genes, used as positive control target gene, and the region of the inactive SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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