Acetylated Lysine Monoclonal Antibody (1C6)/乙酰化赖氨酸单克隆抗体（1C6）

货号：MA1-2021

规格：100 µg

价格：5468

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide sequence surrounding the acetyla

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：1C6

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	10 µg(ChIP)；Assay dependent(DB)；1:100 - 1:500(ICC)；1:100 - 1:500(IF)；3 µg(IP)；1:500 - 1:2000(WB)；Assay Dependent(Array)

产品详细信息MA1-2021 detects human and mouse, and monkey acetylated lysine.MA1-2021 has been used successfully in Western blot, immunoprecipitation, dot blot, ChIP Assay, and immunofluorescence (cells) procedures. By Western blot, this antibody may detect a ladder of acetylated proteins in HeLa cell lysate. In immunofluorescence procedures, MA1-2021 recognizes acetylated lysine in gamma irradiated HeLa cells.The MA1-2021 immunogen is a synthetic peptide sequence surrounding the acetylated lysine 9 of histone H3.靶标信息In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

数据：

Acetylated Lysine Antibody (MA1-2021) in IFImmunofluorescent analysis of lysine acetylated proteins (green) in HeLa cells left untreated (left panels) or treated with 0.3uM Trichostatin A for 16 hours (right panels). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with an Acetyl Lysine monoclonal antibody (Product # MA1-2021) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834). A merged image with nuclei staining (blue) using Hoechst 33342 dye (Product # 62249) is shown on bottom panels. Images were taken on a Thermo Scientific ArrayScan at 20X magnification. Acetylated Lysine Antibody (MA1-2021) in WBWestern blot analysis of lysine acetylated proteins from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours was performed by loading 50 µg of the indicated whole cell lysates per well and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Acetyl Lysine monoclonal antibody (Product # MA1-2021) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

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参考文献：

1. Scientific reportsDNA polymerase ι is acetylated in response to SN2 alkylating agents.2. Biochimica et biophysica actaA SUMO-acetyl switch in PXR biology.3. Scientific reportsDNA polymerase ι is acetylated in response to SN2 alkylating agents.4. Anatomy and cell biologyPoly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity.5. Nucleic acids researchImpaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells.

技术参数

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