SARS-CoV/SARS-CoV-2 Spike Protein Polyclonal Antibody

货号：PA5-112049

规格：0.1mg

价格：4070

产品类型：流式抗体

品牌：Thermo Fisher

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1 µg/mL (ELISA)；1 µg/mL(WB)

产品详细信息The immunogen is located within amino acids 550-600 of SARS-CoV Spike.Predicted reactivity based on immunogen sequence: SARS-CoV2 Spike protein: (identity 65%, homology 88%)靶标信息It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

数据

、SARS-CoV/SARS-CoV-2 Spike Protein Antibody (PA5-112049) in WBWestern Blot analysis of SARS Coronavirus Spike Protein was performed by loading 50 ng per lane of human SARS-CoV-2 Spike S1 recombinant protein. The membrane was blotted with SARS-CoV/SARS-CoV-2 Spike Protein Polyclonal Antibody (Product # PA5-112049), Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL for 1h incubation at RT in 5% NFDM/TBST. A Goat anti-Rabbit IgG HRP conjugate at 1:10000 dilution was used to detect the primary antibody.

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