H2A.ZK7ac Recombinant Rabbit Monoclonal Antibody (RM222)/H2A.ZK7ac重组兔单克隆抗体（RM222）

货号：MA5-24665

规格：100 µg

价格：4130

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetyl-peptide corresponding to Acetyl-Histone H2A

物种：人

宿主：兔

抗体亚型：IgG

克隆号：RM222

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	5 µg/mL(ChIP)；0.2-1 µg/mL(ELISA)；5 µg/mL(ICC)；5 µg/mL(IF)；0.5-2 µg/mL(WB)；0.05-0.5 µg/mL(Array)

产品详细信息This antibody reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

数据：

H2A.ZK7ac Antibody (MA5-24665) in ICCImmunofluorescence analysis of Acetyl-Histone H2A.Z (Lys7) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H2A.Z (Lys7) Rabbit Monoclonal Antibody (RM222) (Product # MA5-24665) at 5 microgram/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents untreated cells with relatively lower expression of Acetyl-Histone H2A.Z (Lys7) (RM222). Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H2A.ZK7ac Antibody (MA5-24665) in ICCImmunofluorescent analysis of HeLa cells treated with sodium butyrate, using Acetyl-Histone H2A.Z (Lys7) Antibody (Product # MA5-24665) (red). Actin filaments have been labeled with FITC-conjugated phalloidin (green).

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