H2A.Zac pan-acetyl (K4,K7,K11) Polyclonal Antibody/H2A.Zac泛乙酰（K4，K7，K11）多克隆抗体

货号：PA5-40095

规格：50 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH-conjugated synthetic peptide corresponding to

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.7 mg/mL	用法：	1 µg/1x10^6 cells(ChIP)；1:200(ELISA)； 1:100(ICC)；1:500(IF)；1:20,000(Array)；Assay dependant(ChIP-Seq)

靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

数据：

H2A.Zac pan-acetyl (K4,K7,K11) Antibody (PA5-40095) in IFImmunofluorescence analysis of Acetyl-Histone H2A.Z (Lys4, Lys7, Lys11) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H2A.Z (Lys4, Lys7, Lys11) Rabbit Polyclonal Antibody (Product # PA5-40095) at 1:100 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells with relatively lower expression of Acetyl-Histone H2A.Z (Lys4, Lys7, Lys11). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H2A.Zac pan-acetyl (K4,K7,K11) Antibody (PA5-40095) in ChIPChIP assays were performed on HeLa cells using a Acetyl-Histone H2A.Z (Lys4 + Lys7 +Lys11) polyclonal antibody (Product # PA5-40095) and optimized primer pairs for qPCR. ChIP was performed on sheared chromatin from 1 million HeLaS3 cells and a titration of the antibody consisting of 1, 2, 5 and 10 µg per ChIP experiment was analyzed. IgG (2 µg/IP) was used as negative IP control. QPCR was performed on primers specific for the promoters of the ACTB and EIF4A2 genes, used as positive control targets and for the coding region of the MYT1 gene, used as a negative control target. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H2A.Z is present at active promoters.

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