H2BK15ac Polyclonal Antibody/H2BK15ac多克隆抗体

货号：PA5-40103

规格：50 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH-conjugated synthetic peptide corresponding to

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	2.1 mg/mL	用法：	2 µg(ChIP)；1:29,700(ELISA)； 1:500(IF)；1:500(WB)；1:20,000(Array)；Assay dependant；(ChIP-Seq)

靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

数据：

H2BK15ac Antibody (PA5-40103) in IFHeLa cells were stained with the anti-H2BK15ac antibody (Product # PA5-40103) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK15ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. H2BK15ac Antibody (PA5-40103) in ChIPChIP assays were performed on human HeLa cells using a Acetyl-Histone H2B (Lys15) polyclonal antibody (Product # PA5-40103) and optimized PCR primer sets for qPCR. ChIP was performed with sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 µg per ChIP experiment was analyzed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis)。

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