H2BK20ac Recombinant Rabbit Monoclonal Antibody (RM235)/H2BK20ac重组兔单克隆抗体（RM235）

货号：MA5-24698

规格：100 µg

价格：4130

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetyl-peptide corresponding to Acetyl-Histone H2B

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：RM235

荧光染料：其它

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；0.2-1 µg/mL(ELISA)；5 µg/mL(ICC)；5 µg/mL(IF)；1 µg/mL(WB)；0.1-0.5 µg/mL(Array)

产品详细信息This antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

数据：

H2BK20ac Antibody (MA5-24698) in IFImmunofluorescence analysis of Acetyl-Histone H2B (Lys20) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody (RM235)(Product # MA5-24698) at 5 µg/mL in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells with relatively lower expression of Acetyl-Histone H2B (Lys20). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H2BK20ac Antibody (MA5-24698) in ChIPEnrichment of endogenous Acetyl-Histone H2B (Lys20) protein at specific gene loci using Anti-Acetyl-Histone H2B (Lys20) Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Acetyl-Histone H2B (Lys20) Rabbit Monoclonal Antibody (Product # MA5-24698, 3 µg) on sheared chromatin from 2 million Sodium butyrate-treated HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the promoters of CFOS, GAPDH used as positive, and MYOD, SAT2 satellite repeats used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

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