H4K20ac Recombinant Polyclonal Antibody (2HCLC)/H4K20ac重组多克隆抗体（2HCLC）

货号：710810

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetylated peptide (Lys20) corresponding to human

物种：人

宿主：兔

抗体亚型：IgG

克隆号：2HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/mL(ICC)；1 µg/mL(IF)；1-2 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producinghigher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H4K20ac Antibody (710810) in IFImmunofluorescence was performed on fixed and permeabilized Hela cells for detection of Histone H4K20ac using Anti-Histone H4K20ac Recombinant Rabbit Polyclonal Antibody (Product # 710810, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H4K20ac protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H4K20ac. Panel e) represents control cells with no primary Antibody to assess background. H4K20ac Antibody (710810) in ARRAYThe specificity of the antibody, Anti-Acetyl-Histone H4 (Lys20) Recombinant Rabbit Polyclonal Antibody (Product # 710810, 1:2000 dilution) to Histone H4K20ac peptide was confirmed using MODified™ Histone Peptide Array (Active Motif, 13001). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used as the secondary antibody. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). The dot blot data obtained (lower panel) was analyzed using Array Analyze software as per the manufacturer's instructions (top panel).

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