H3K4me2 Recombinant Polyclonal Antibody, ChIP-Verified/H3K4me2重组多克隆抗体，ChIP验证

货号：710796,710796-20UG

规格：100 µg,20 µg

价格：4809,1535

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Methylated peptide (Lys4) corresponding to human H

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-5 µg(ChIP)；1 µg/mL(ICC)；1 µg/mL(IF)；0.5-1 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producinghigher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K4me2 Antibody (710796) in IFImmunofluorescence was performed on fixed and permeabilized Hela cells for detection of Histone H3K4me2 using Anti-Histone H3K4me2 Recombinant Rabbit Polyclonal Antibody (Product # 710796, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H3K4me2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b, and c clearly demonstrating nuclear localization of Histone H3K4me2. Panel e) represents control cells with no primary Antibody to assess background. H3K4me2 Antibody (710796) in ChIPK-MetStat Panel, SNAP-ChIP™ Spike-in (Product # A47356), a proprietary technology developed byEpiCypher™was used to analyze the performance of H3K4me2 antibody (Product # 710796) in ChIP. SNAP-ChIP panels consist of a pool of DNA-barcoded recombinant nucleosomes harboring histone PTMs that are spiked-in to a ChIP reaction to assess efficiency and specificity of the antibody. The K-MetStat panel includes an unmodified control plus nucleosomes containing histones with mono, di and tri-methyl forms of lysine residues on histone H3 and H4 as shown on the x-axis. Recovery of each unique DNA-barcoded nucleosome is quantified to determine how much of each PTM is immunoprecipitated in the ChIP reaction (for more information seereference). H3K4me2 antibody was tested in native ChIP with 3 µg HEK-293 cell chromatin and 3 µg antibody. Specificity (left Y-axis) was determined by qPCR to each modified nucleosome in the panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded nucleosome target immunoprecipitated relative to Input. All bars represent mean ± SEM.

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