H3K4me3 Polyclonal Antibody/H3K4me3多克隆抗体

货号：49-1005

规格：50 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Raised against the region of the histone H3 contai

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1.4 mg/mL	用法：	1:1000(ELISA)；1:200(ICC)；1:200(IF)；1:1000(WB)；0.25 µg/mL(Array)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K4me3 Antibody (49-1005) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys4) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # 49-1005) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K4me3 Antibody (49-1005) in IFHeLa cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilizes the morphology of the cells and permeabilizes membranes. (A) Cells were labeled with the H3K4me3 Polyclonal Antibody, Rabbit (diluted 1:200 and incubated for 1 hour at room temperature) followed by a FITC-labeled goat anti-rabbit secondary antibody. (B) Nuclei were stained using the DNA-specific stain DAPI. Both, the H3K4me3 Polyclonal Antibody, Rabbit and DAPI produced clear nuclear staining.

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参考文献：

1. OncotargetThe Jumonji-domain histone demethylase inhibitor JIB-04 deregulates oncogenic programs and increases DNA damage in Ewing Sarcoma, resulting in impaired cell proliferation and survival, and reduced tumor growth.2. OncotargetTumorigenicity of Ewing sarcoma is critically dependent on the trithorax proteins MLL1 and menin.3. OncotargetA bivalent promoter contributes to stress-induced plasticity of CXCR4 in Ewing sarcoma.4. NatureReplication stress is a potent driver of functional decline in ageing haematopoietic stem cells.

技术参数

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