Human GAPDH ELISA Kit ELISA试剂盒,Human GAPDH ELISA Kit/人GAPDH ELISA试剂盒

2024-10-23

Human GAPDH ELISA Kit/人GAPDH ELISA试剂盒

货号:EH207RB,EH207RBX5,EH207RBX10

规格:96 tests,5X96 tests,10X96 tests

价格:6641,29884,54564

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.82 ng/mL

检测范围0.82-200 ng/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameGAPDH
Protein aliasesaging-associated gene 9 protein, epididymis secretory protein Li 278, epididymis secretory sperm binding protein Li 162eP, GAPDH, GAPDH-2, Glyceraldehyde-3-phosphate dehydrogenase, testis-specific, Peptidyl-cysteine S-nitrosylase GAPDH, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCDABP0047, G3PD, GAPD, GAPD2, GAPDH, GAPDH-2, GAPDH2, GAPDHS, GAPDS, HEL-S-162eP, HEL-S-278, HSD-35, HSD35, OK/SW-cl.12
Gene ID(Human) 2597,(Human) 26330
Gene symbolGAPDH, GAPDHS
UniProt ID(Human) P04406,(Human) O14556
Human GAPDH quantitates human GAPDH in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human GAPDH.人GAPDH定量测定血清,血浆,上清液中的人GAPDH。 该检测试剂盒将专门识别天然和重组人GAPDH。Target information 靶标信息GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.GAPDH(甘油醛-3-磷酸脱氢酶)是一种通常被认为参与糖酵解的催化酶。 GAPDH以相同的37kDa亚基的四聚体形式存在,并在NADPH存在下催化1,3-双磷酸甘油酯可逆还原为3-磷酸甘油醛。除了在糖酵解中起关键作用外,GAPDH广泛表达并显示与其糖酵解功能无关的其他活性。据报道,GAPDH参与DNA复制,DNA修复,核RNA输出,膜融合和微管捆绑的过程。研究提供了证据,证明GAPDH在凋亡中观察到的基因表达中起着必不可少的作用,并且是与年龄相关的神经退行性疾病的细胞表型的一部分。此外,GAPDH还参与了其他细胞过程,包括膜融合和癌症中的神经元凋亡。据报道,GAPDH与多种其他蛋白结合,包括淀粉样蛋白前体蛋白,引起某种形式的阿尔茨海默氏病(AD)的突变以及亨廷顿蛋白的多谷氨酰胺束,其蛋白产物异常形式是亨廷顿氏病的病因。 GAPDH,肌动蛋白和微管蛋白之间的关联也有报道。由于GAPDH在大多数组织中均以高水平表达,因此在蛋白质印迹分析中可用作蛋白质负载控制。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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