PRMT1 Polyclonal Antibody

货号：PA5-17299

规格：100 µL

价格：4532

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the N-terminus

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:100-1:200(ICC) ; 1:100-1:200(IF);1:50(IP);1:1000(WB)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other PRMT proteins. 靶标信息PRMT1 is a member of the protein arginine N-methyltransferase (PRMT) family. Arginine methylation is an irreversible post translational modification which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Increased expression of PRMT1 may play a role in various types of cancer.

数据：

PRMT1 Antibody (PA5-17299) in IFImmunofluorescence analysis of PRMT1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRMT1 Polyclonal Antibody (Product # PA5-17299) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. PRMT1 Antibody (PA5-17299) in WBKnockdown of PRMT1 was achieved by transfecting HCT 116 cells with PRMT1 specific siRNAs (Silencer® select Product # s6918; s6917). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the PRMT1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PRMT1 Polyclonal Antibody (Product # PA5-17299, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PRMT1.

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