VRK1 Monoclonal Antibody (1F6)

货号：MA1-828

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant fusion protein GST-VRK1.

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1F6

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100(ICC);1:100(IF)；1:100 - 1:500（IHC）;1:500(WB)

产品详细信息MA1-828 detects VRK1 from human and mouse samples.MA1-828 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry applications. For Western blotting, it is recommended to block with 3% BSA (not milk).The MA1-828 immunogen is recombinant fusion protein GST-VRK1. 靶标信息Human vaccinia-related kinase 1 (VRK1) is a new kinase related to a poxvirus kinase, and more distantly to the casein kinase 1 family. VRK1 shows strong autophosphorylating activity in several Ser and Thr residues. VRK has also been found to be an upstream regulator of p53 that belongs to a new signaling pathway.

数据：

VRK1 Antibody (MA1-828) in IF Immunofluorescence analysis of VRK1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VRK1 (1F6) Mouse Monoclonal Antibody (Product # MA1-828) at 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. VRK1 Antibody (MA1-828) in WB Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK 293 (Lane 2), MCF7 (Lane 3), and Jurkat (Lane 4). The blot was probed with Mouse Anti-VRK1 Monoclonal Antibody (Product # MA1-828, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 48 kDa band corresponding to VRK1 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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