组蛋白甲基化修饰,H3K4,多克隆抗体,ASH1 Polyclonal Antibody

2024-10-24

ASH1 Polyclonal Antibody

货号:A301-749A

规格:100 µL

价格:6082

产品类型:标签抗体

品牌:Thermo Fisher

抗原:Between 2919 and 2969

物种:人/小鼠

宿主:兔

抗体亚型:IgG

荧光染料:其它

类型:一抗同型对照:
浓度: 1 mg/mL用法:1:1,000-1:5,000(IHC);2-5 µg/mg lysate(IP)1:2,000-1:10,000(WB)
产品详细信息The recommended shelf life for this product is 1 year from date of receipt.Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 靶标信息ASH1 (ASH1L) is a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. Diseases associated with ASH1 include Mental Retardation, Autosomal Dominant 52 and Pulpitis.
数据:

ASH1 Antibody (A301-749A) in IHC Detection of human ASH1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASH1 (Cat. No. A301-749A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

ASH1 Antibody (A301-749A) in WB Detection of human ASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH1 antibody A301-749A (lot A301-749A-3) used for IP at 3 µg per reaction. ASH1 was also immunoprecipitated by rabbit anti-ASH1 antibody A301-748A. For blotting immunoprecipitated ASH1, A301-749A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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