Human Thioredoxin-1 ELISA Kit ELISA试剂盒,Human Thioredoxin-1 ELISA Kit/人Thioredoxin-1 ELISA试剂盒

2024-10-24

Human Thioredoxin-1 ELISA Kit/人Thioredoxin-1 ELISA试剂盒

货号:EH451RB,EH451RBX5,EH451RBX10

规格:96 tests,5X96 tests,10X96 tests

价格:6641,29884,57271

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

0.2 ng/mL

检测范围0.2-50 ng/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein nameThioredoxin 1
Protein aliasesADF, ATL-derived factor, SASP, Surface-associated sulphydryl protein, testicular tissue protein Li 199, Thioredoxin, thioredoxin delta 3, Trx, TXN delta 3

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesTRDX, TRX, TRX1, TXN
Gene ID(Human) 7295
Gene symbolTXN
UniProt ID(Human) P10599
Human Thioredoxin-1 quantitates human Thioredoxin-1 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Thioredoxin-1.人硫氧还蛋白-1定量检测血清,血浆,上清液中的人硫氧还蛋白-1。 该检测试剂盒将专门识别天然和重组人硫氧还蛋白-1。Target information 靶标信息Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.硫氧还蛋白(Trx)是具有氧化还原酶活性的小型多功能蛋白质,在所有活细胞中普遍存在。 Trx在保守的Cys-Gly-Pro-Cys活性位点内包含氧化还原活性的二硫键/二硫醇基团。保守的活性中心中的两个半胱氨酸残基可被氧化形成分子内二硫键。 Trx还原酶以NADPH作为电子供体,催化氧化的Trx中活性位点二硫键的还原。还原的Trx是核糖核苷酸还原酶的氢供体,是DNA合成所必需的酶,也是有效的一般蛋白质二硫键还原酶,在生长和氧化还原调节中具有多种功能。 Trx还原的特定蛋白质二硫键靶标包括蛋白质二硫键异构酶(PDI)和许多转录因子,例如p53,NF-kB和AP-1(T1-151)。 Trx还能够去除H2O2,特别是当它与蛋氨酸亚砜还原酶或过氧化物环氧还蛋白的几种同工型结合时。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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